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  • Title: Topographical studies on intestinal microvillous leucine beta-naphthylamidase on the outer membrane surface.
    Author: Takesue Y, Nishi Y.
    Journal: J Membr Biol; 1978 Mar 20; 39(4):285-96. PubMed ID: 76678.
    Abstract:
    The location of leucine beta-naphthylamidase on the outer surface of the microvillous membrane of rabbit small intestine was examined by analyzing the interaction of antibodies against leucine beta-naphthylamidase or another microvillous enzyme, sucrase-isomaltase complex, with microvillous vesicles having different relative amounts of these enzymes, in respect to vesicle agglutination, inhibition of enzyme activity, and electron-microscopic morphology. The results obtained indicate that leucine beta-naphthylamidase, or at least its antigenic sites, protrude about 10 nm from the outer surface of the microvillous membrane.
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