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  • Title: Purification of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi by affinity chromatography.
    Author: Labriola C, Sousa M, Cazzulo JJ.
    Journal: Biol Res; 1993; 26(1-2):101-7. PubMed ID: 7670520.
    Abstract:
    The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity.
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