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  • Title: Human seminal plasma beta-microseminoprotein: its purification, characterization, and immunohistochemical localization.
    Author: Ohkubo I, Tada T, Ochiai Y, Ueyama H, Eimoto T, Sasaki M.
    Journal: Int J Biochem Cell Biol; 1995 Jun; 27(6):603-11. PubMed ID: 7671139.
    Abstract:
    beta-Microseminoprotein was very efficiently purified from human seminal plasma with only three steps including DEAE-Sephacel and Zinc-chelate Sepharose CL-6B column chromatography. The purified protein was a non-glycoprotein with a molecular weight (M(r)) of 19,000 and 17,000 on gel filtration and reduced SDS-PAGE, respectively. The protein gave six bands from M(r) 15,600 to 25,500 on non-reduced SDS-PAGE. The characterization including the molecular weight, amino acid sequence of N-terminus and concentrations in various body fluids is discussed. Furthermore, the immunohistochemical localization of the protein among various human tissues is demonstrated.
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