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  • Title: Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.
    Author: Warner JR, Morgan SA, Shulman RW.
    Journal: J Bacteriol; 1976 Mar; 125(3):887-91. PubMed ID: 767330.
    Abstract:
    A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine is reduced by half within 20 s. During that period there is a substantial expansion of the pool. A logarithmically growing cell in synthetic medium contains about 2 X 10(6) molecules of S-adenosylmethionine, of which only 10% is used for the methylation of ribonucleic acid molecules.
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