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  • Title: Phase partitioning detects differences between phospholipase-released forms of alkaline phosphatase--a GPI-linked protein.
    Author: Raymond FD, Moss DW, Fisher D.
    Journal: Biochim Biophys Acta; 1993 Feb 13; 1156(2):117-22. PubMed ID: 7678987.
    Abstract:
    A number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. We describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. The procedures avoid the complications of micelle-formation involving hydrophobic molecules that may arise with detergent-based partition systems and can clearly distinguish between inositol-specific phospholipase C and D activities.
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