These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Peptide-induced memory (IgG) response, cross-reactive with native proteins, requires covalent linkage of a specific B cell epitope with a T cell epitope.
    Author: Zegers ND, van Holten C, Claasen E, Boersma WJ.
    Journal: Eur J Immunol; 1993 Mar; 23(3):630-4. PubMed ID: 7680611.
    Abstract:
    In order to raise antibodies synthetic peptides are often coupled to a carrier protein to provide the necessary T cell determinants. Alternatively, a short synthetic determinant with a distinct sequence motif which can be presented by major histocompatibility complex (MHC) class II to T cells, can be linked directly to a B cell epitope. Recently, it has been suggested that covalent linkage between a class II-presentable T helper peptide and a B cell epitope is not required to induce antibodies against a B cell determinant (Sarobe et al., Eur. J. Immunol. 1991. 21: 1555). Therefore, we investigated the ability of an H-2d-restricted T cell determinant (AA 111-120 FERFEIFPKEK) from the influenza virus hemagglutinin, to support B cell responses to different proven B cell determinant peptides, derived from human alpha 1-antitrypsin. Antibodies against B cell epitopes crossreactive with native alpha 1-antitrypsin could be raised only when these B epitope peptides were covalently coupled to the T cell determinant through a peptide bond. No antibodies were raised against the B cell epitope when the free peptides (T and B cell epitopes) were just mixed or when the T cell epitope was conjugated via m-maleimidobenzoyl succinimide ester or bis-maleimidohexane to the B cell determinant. Antibodies against the T cell determinant were raised in all cases, regardless of the mode of presentation: just mixed with or covalently coupled to the B cell determinant. The results indicate that a covalent bond between T cell and B cell determinants in general is needed to induce anti B cell determinant antibodies cross-reactive with the native protein.
    [Abstract] [Full Text] [Related] [New Search]