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  • Title: [The participation of polymerized actin in maintaining the spatial organization of the oocyte in the clawed toad and its detection in the deep regions of the ooplasm].
    Author: Riabova LV, Vasetskiĭ SG.
    Journal: Ontogenez; 1993; 24(2):55-61. PubMed ID: 7683782.
    Abstract:
    Microfilaments were shown to be involved in maintaining spatial organization of the Xenopus laevis oocyte by using injections of DNAse I, which selectively destroys F-actin. An original method has been proposed for visualization of polymerized actin at the electron microscope level. Standard histological sections (5-6 um) were deparaffinized, treated with gold-labelled phalloidin, dehydrated, and embedded in epoxy resins using gelatin capsules placed onto sections. After resin polymerization, the section separated from the slide in liquid nitrogen was cut in ultrathin sections on an ultratome. This method allows to by-pass the cutting on an ultracryotome and to study the distribution of polymerized actin in any region of the oocyte at the electron microscope level. The method allows to make the light immunohistochemistry data more precise. We have shown using this method the presence of F-actin not only in the cortex, as was established earlier, but also in deep layers of the ooplasm. Some aspects of the intracellular matrix organization are discussed.
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