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  • Title: Phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase activities in Md 66 insect cell line.
    Author: Yadwad VB, Downer RG.
    Journal: Arch Int Physiol Biochim Biophys; 1993; 101(3):203-5. PubMed ID: 7691214.
    Abstract:
    Phosphatidylinositol kinase (PI kinase) and phosphatidylinositol 4-phosphate kinase (PIP kinase) activities in an insect cell line (Md 66) were studied by measuring the incorporation of 32P from [gamma-32P] ATP into phosphatidylinositol 4-phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate (PIP2) respectively. The apparent Km values of phosphatidylinositol kinase and phosphatidylinositol 4-phosphate kinase for ATP were 78.5 microM and 71.7 microM respectively. PIP kinase activity was enhanced by GTP analogue guanosine 5-O(thio)triphosphate (GTP gamma S).
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