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  • Title: Identification and regulation of whole-cell Cl- and Ca(2+)-activated K+ currents in cultured medullary thick ascending limb cells.
    Author: Lu L, Markakis D, Guggino WB.
    Journal: J Membr Biol; 1993 Aug; 135(2):181-9. PubMed ID: 7692066.
    Abstract:
    The whole-cell patch-clamp technique has been used to study membrane currents in cultured rabbit medullary thick ascending limb (MTAL) epithelial cells. A Ca(2+)-activated K+ current was characterized by its voltage-dependent and Ca(2+)-dependent properties. When the extracellular K+ ion concentration was increased from 2 to 140 mM, the re-reversal potential (Ek) was shifted from -85 to 0 mV with a slope of 46 mV per e-fold change. The Ca(2+)-activated K+ current is blocked by charybdotoxin (CTX) in a manner similar to the apical membrane Ca(2+)-activated K+ channel studied with the single channel patch-clamp technique. The results suggest that the Ca(2+)-activated K+ current is the predominant, large conductance and Ca(2+)-dependent K+ pathway in the cultured MTAL cell apical membrane. The biophysical properties and physiological regulation of a Cl- current were also investigated. This current was activated by stimulation of intracellular cAMP using forskolin and isobutyl-1-methylxanthine (IBMX). The current-voltage (I-V) relationship of the Cl- current showed an outward-rectifying pattern in symmetrical Cl- solution. The Cl- selectivity of the whole-cell current was confirmed by tail current analysis in different Cl- concentration bath solutions. Several Cl- channel blockers were found to be effective in blocking the outward-rectifying Cl- current in MTAL cells. The cAMP-dependent Cl- transport in MTAL cells was further confirmed by measuring changes in the intensity of Cl- sensitive dye using fluorescence microscopy.(ABSTRACT TRUNCATED AT 250 WORDS)
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