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  • Title: Preparation, optimization and characterization of a polyphenylalanine synthetizing system from Agrobacterium tumefaciens.
    Author: Alegre C, Iglesias R, Ferreras JM, Carbajales ML, Girbés T.
    Journal: Cell Mol Biol (Noisy-le-grand); 1993 Sep; 39(6):575-81. PubMed ID: 7693097.
    Abstract:
    A very active cell-free translation system was prepared from Agrobacterium tumefaciens, a bacterium broadly used to transfect plant cells to introduce foreign genes and one that produces tumours in plants. Once optimized for Mg2+, NH4+, high speed supernatant S 370, purified ribosomes and time, the system translates polyuridylic acid very efficiently. A. tumefaciens purified ribosomes were inhibited in vitro by several well-known translational inhibitors including some ribosome-inactivating proteins. Treatment of A. tumefaciens purified ribosomes with type 1-RIP crotin 2 lead to the depurination of the 23S rRNA which, upon treatment with acid aniline, released a diagnostic RNA fragment of about 235 nucleotides.
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