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  • Title: Liquid chromatographic determination of salbutamol and clenbuterol residues in swine serum and muscle.
    Author: Tsai CE, Kondo F.
    Journal: Microbios; 1994; 80(325):251-8. PubMed ID: 7700164.
    Abstract:
    A simple and rapid method has been developed for determination of residual clenbuterol and salbutamol in swine serum and muscle using high-performance liquid chromatography. In a Nova-pak C18 column, the drug peaks were separated by a gradient using two mobile phases [acetonitrile:water (15:85, v/v) and acetonitrile:water (30:70, v/v)] for salbutamol and clenbuterol, respectively. A photodiode array set at 196 nm for salbutamol and 210 nm for clenbuterol, -0.01 to +0.2 AU, ambient temperature and flow rate of 1.0 ml/min, were used. The retention times were 5.4 and 21 min for salbutamol and clenbuterol, respectively. The detection limits for this method were 0.1 and 0.2 microliter/ml for salbutamol and clenbuterol, respectively. Average recoveries (0.1, 0.25, 0.5, 1.0 ppm spiked level) were 86.7 +/- 1.7% for salbutamol; 80.0 +/- 2.0% for clenbuterol spiked in serum; 64.2 +/- 8.9% for salbutamol; 62.9 +/- 4.2% for clenbuterol spiked in the muscle, respectively. The minimum detectable amount was 0.1 ppm, based on a spiked sample extract.
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