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  • Title: [A synthetic medium containing growth factors for the cultivation of producer cell lines].
    Author: Kiziun SM, Afanasenko GA.
    Journal: Tsitologiia; 1994; 36(8):822-8. PubMed ID: 7701613.
    Abstract:
    A synthetic medium with low serum contents has been composed. As basic substitutes for serum a mixture containing a fibroblast growth factor, insulin, albumin and fibronectin is proposed. It allows to carry out a long-term cultivation of RH-PA cells, which are producers of a plasminogen activator, both in static conditions and on microcarriers. The suitability of the suggested medium for cultivation of other biotechnologically important cell lines (SPEV, NIH 3T3, Vero, CHO) is demonstrated.
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