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  • Title: [Polychromatic method of simultaneously demonstrating all the structural components of a peripheral nerve].
    Author: Dolishniĭ NV, Mel'man EP.
    Journal: Arkh Anat Gistol Embriol; 1978 Feb; 74(2):99-102. PubMed ID: 77154.
    Abstract:
    To study neuro-vessel relations in the nerve conductive system, a combined method revealing myelinated and non-myelinated nerve fibers and blood vessels was suggested. The method includes the following stages of material treatment: injection of the nerve blood vessels with chloroform emulsion of Paris blue (5--10 g of the dye in 100 g of the solvant); the straightened nerves about 5 mm long are fixed in 12% neutral formalin; etching in the solution of chromium solts; embedding in paraffin and preparation of thin sections (3--5 mcm); staining after Masson's method applied for collagen fiber staining) in the author's modification and mounting in balsam. In the preparations, the axons of the nerve fibers are seen blue--purple, myelin sheaths--light red, connective tissue elements--violet and blood vessels are seen as dark blue rings.
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