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  • Title: [The influence of different cryopreservation buffers on the fertilizing ability of human sperm "in vitro". II. Evaluation of the fertilizing potential of human sperm after cryopreservation].
    Author: Jedrzejczak P, Pisarski T, Kurpisz M.
    Journal: Ginekol Pol; 1994 Aug; 65(8):456-61. PubMed ID: 7721154.
    Abstract:
    We tried to define a potential ability of human spermatozoa for fertilization after 3 month preservation of samples in a liquid nitrogen. The functional test were applied: penetration of sperm to cervical mucus ("Penetrak") and sperm penetration to hamster zona-free oocytes. We would like to emphasize that presented way of cryo-preservation in 3 selected media with fast cooling of samples allow to maintain fertilizing ability of spermatozoa and subsequent insemination.
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