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  • Title: Pulsed-field gel analysis of 5S and satellite DNA in barley.
    Author: Röder MS, Sorrells ME, Tanksley SD.
    Journal: Genome; 1995 Feb; 38(1):153-7. PubMed ID: 7729679.
    Abstract:
    Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
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