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  • Title: The requirement for molecular chaperones in lambda DNA replication is reduced by the mutation pi in lambda P gene, which weakens the interaction between lambda P protein and DnaB helicase.
    Author: Konieczny I, Marszalek J.
    Journal: J Biol Chem; 1995 Apr 28; 270(17):9792-9. PubMed ID: 7730358.
    Abstract:
    During the initiation of lambda DNA replication, the host DnaB helicase is complexed with phage lambda P protein in order to be properly positioned near the ori lambda-lambda O initiation complex. However, the lambda P-DnaB interaction inhibits the activities of DnaB. Thus, the concerted action of bacterial heat shock proteins, DnaK, DnaJ, and GrpE, is required to activate the helicase. Wild-type phage lambda cannot grow on the E. coli dnaB, dnaK, dnaJ, and grpE mutants. However, lambda phage with a mutation pi in the lambda P gene, is able to produce progeny in these mutants as well as in the wild-type bacteria. Purified mutant lambda pi protein reveals a much lower affinity to DnaB than wild-type lambda P, and the lambda pi-DnaB complex is unstable. Also, a very low concentration of DnaK protein is sufficient to activate the helicase in a replication system based on lambda dv dsDNA. In that system, the mutant DnaK756 protein, inactive in the lambda P-dependent replication, revealed its activity in the lambda pi-dependent reaction. The lambda O-lambda P-dependent replication system based on M13 ssDNA efficiently replicates DNA in the absence of any chaperone protein, unless lambda P is substituted by the lambda pi mutant protein. Data presented in this paper explain why lambda pi phage is able to grow on wild-type and dnaK756 bacteria.
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