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  • Title: Freeze etch morphology of outer membrane mutants of Escherichia coli K12.
    Author: Verkleij AJ, Lugtenberg EJ, Ververgaert PH.
    Journal: Biochim Biophys Acta; 1976 Mar 19; 426(3):581-6. PubMed ID: 773430.
    Abstract:
    Freeze etching showed that the loss of each of the major outer membrane proteins b, c or d in mutants of Escherichia coki K12 does not influence the morphology of fracture faces of the outer membrane. Mutants that possess a heptose-deficient lipopolysaccharide and which in addition are deficient in one or more major outer membrane proteins exhibit a reduction in the number of intramembranous particles of the outer membrane. Moreover it was shown that lipid phase transitions induce a lateral lipid protein separation in the outer membrane, similar to that found in the cytoplasmic membrane.
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