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  • Title: Single-strand conformation polymorphism analysis by perpendicular temperature-gradient gel electrophoresis.
    Author: Sugano K, Fukayama N, Ohkura H, Shimosato Y, Yamada Y, Inoue T, Sekiya T, Hayashi K.
    Journal: Electrophoresis; 1995 Jan; 16(1):8-10. PubMed ID: 7737095.
    Abstract:
    Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor suppressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change--depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.
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