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  • Title: [Synthesis of estradiol by endometrial stroma cells under the influence of epidermal growth factor (EGF) in vitro].
    Author: Tomaszewski J, Kotarski J.
    Journal: Ginekol Pol; 1994 Nov; 65(11):638-41. PubMed ID: 7737543.
    Abstract:
    The endometrial samples were obtained from 13 operated women in the proliferative phase of menstrual cycle. The tissue culture of endometrial stromal cells was established. After the cells were growth to the confluency, EGF was added to dishes of study group to the final concentrations of 5 ng/ml. Culture media of study and control groups were collected every 48, 96, 144, 192 hours and lyophylized. The estradiol level was measured in culture media by RIA. The mean estradiol concentrations in culture media of endometrial stromal cells decreased during the time of experiment in the control: 318 pg/ml, 276 pg/ml, 186 pg/ml and 110 pg/ml and study group respectively: 339 pg/ml, 279 pg/ml, 169 pg/ml and 129 pg/ml. There was no significant influence of EGF (5 ng/ml) on estradiol level in culture media in each time intervals.
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