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  • Title: Production and characterization of specific bovine plasminogen monoclonal antibodies.
    Author: Dupont D, Labiau S, Grosclaude J, Collin JC.
    Journal: Hybridoma; 1994 Dec; 13(6):543-7. PubMed ID: 7737679.
    Abstract:
    A collection of monoclonal antibodies was raised against bovine plasminogen by immunizing mice with purified plasminogen. More than 300 positive clones were obtained in 1 fusion experiment (1300 hybrids tested). Four types of antibodies were characterized through their relative binding to plasmin and plasminogen in ELISA. About one-third was strictly specific to plasminogen; the selectivity of this group was confirmed by immunoblots and BIA-core analysis. Cross-reactivity against horse, human, pig, sheep, rabbit, and bovine plasminogens was tested; 41% were strictly bovine specific.
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