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  • Title: Generation of an antibody to HLA-G in transgenic mice and demonstration of the tissue reactivity of this antibody.
    Author: Chumbley G, King A, Gardner L, Howlett S, Holmes N, Loke YW.
    Journal: J Reprod Immunol; 1994 Dec; 27(3):173-86. PubMed ID: 7738907.
    Abstract:
    A method was devised to generate antibodies against the non-classical class I HLA-G antigen. This consisted of immunising HLA-A2/beta 2m double transgenic mice with HLA-G transfected into mouse Ltk- cells. A polyclonal antibody was obtained which appears to be specific for HLA-G. The staining pattern of this antibody was restricted solely to all populations of extravillous trophoblast. No fetal tissues reacted with this antibody, including those where HLA-G mRNA has been demonstrated, such as fetal eye, thymus and liver. This study confirms that HLA-G is a trophoblast-specific protein, although it remains a possibility that the technique of immunohistology is not sufficiently sensitive to detect low level HLA-G antigen expression in non-trophoblast tissues.
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