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  • Title: Biochemical characterization of a bovine oviduct-specific sialo-glycoprotein that sustains sperm viability in vitro.
    Author: Satoh T, Abe H, Sendai Y, Iwata H, Hoshi H.
    Journal: Biochim Biophys Acta; 1995 Apr 28; 1266(2):117-23. PubMed ID: 7742375.
    Abstract:
    A bovine oviduct-specific glycoprotein (BOGP) that sustained the viability of bovine spermatozoa in vitro was purified from an extract of bovine oviducts. The amino-terminal amino acid sequence of BOGP was found to be a homologous with that of oviductin, a protein from hamster that was recently characterized by Mallete and Bleau (1993: Biochem. J. 295, 437-445). Purified BOGP was characterized as a sialo-glycoprotein containing N-linked and O-linked sialo-oligosaccharides side chains with galactose, mannose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, fucose and sialic acids in its core protein (57 kDa). Intact BOGP has a wide range of isoelectric points (pIs) from 6.5 to 3.0 but a narrow range of molecular masses around 95 kDa. On isoelectric focusing of neuraminidase-treated BOGP (AS-BOGP), a narrow band with a pI of 9.3 was observed, and the ability of AS-BOGP to maintain sperm viability was negligible. We propose that BOGP is a mucin-type sialo-glycoprotein with a molecular mass of 72 kDa that contains one N-linked and approx. 15 O-linked sialo-oligosaccharide chains. These side chains appear to be important for the maintenance of sperm viability.
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