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  • Title: A simple fluorometric assay for quantifying the adhesion of tumour cells to endothelial monolayers.
    Author: Price EA, Coombe DR, Murray JC.
    Journal: Clin Exp Metastasis; 1995 May; 13(3):155-64. PubMed ID: 7750203.
    Abstract:
    A static adhesion assay employing 6-carboxy-3',6'-diacetylfluorescein (6-CFDA) as a fluorescent marker has been developed to study the interactions of tumour cell lines with endothelial monolayers. This assay allows simple, safe quantification of cell-cell adhesion using living cells. It has been used to demonstrate that the integrin adhesion molecule VLA-4 mediates the attachment of RPMI-7951 melanoma cells to human umbilical vein endothelial cells (HUVEC) which have been activated by TNF alpha. In addition, MDA-MB-231 breast adenocarcinoma cells display greater adhesion to microvessel endothelial cells than to large vessel endothelial cells.
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