These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The role of membrane-associated iron-binding complex in intestinal iron absorption in the rat.
    Author: Orimo H, Hisayasu S, Hirai Y, Yoshino Y.
    Journal: J Nutr Sci Vitaminol (Tokyo); 1994 Dec; 40(6):511-22. PubMed ID: 7751970.
    Abstract:
    A membrane-associated iron-binding complex was isolated from rat intestinal mucosa during iron absorption. Two 59Fe peaks (peaks 1 and 2) were separated on Sepharose 6B gel filtration from detergent solubilized 20,000 x g precipitate of upper intestinal mucosal homogenate after administration of 59Fe-labelled ferrous materials. Peak 1 was a membrane iron-binding complex whose apparent molecular weight was over 10(6) Da estimated by gel filtration, while peak 2 was identified as ferritin. Three major bands were detected on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of peak 1. The treatment of mucosal homogenate with 5 mM ethylenediaminetetraacetic acid released 59Fe binding from peak 1. Incorporation of 59Fe to peak 1 showed the maximum at 1 h and then reduced, while [59Fe]ferritin showed reciprocal behavior, which suggested that peak 1 may be a rapid turnover iron pool and transfer 59Fe to ferritin. Peak 1 was also isolated from the brush border membrane and showed similar SDS-PAGE pattern to that from the 20,000 x g precipitate of mucosal homogenate. Western blot analysis did not reveal immunoreactive transferrin in peak 1. Those findings suggest that peak 1 may be a non-ferritin, non-transferrin iron-binding complex located on the brush border membrane and accept iron from the intestinal canal during iron absorption.
    [Abstract] [Full Text] [Related] [New Search]