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  • Title: Atomic force microscopy of cholera toxin B-oligomers bound to bilayers of biologically relevant lipids.
    Author: Mou J, Yang J, Shao Z.
    Journal: J Mol Biol; 1995 May 05; 248(3):507-12. PubMed ID: 7752220.
    Abstract:
    Cholera toxin B-oligomer was imaged by atomic force microscopy (AFM) on biologically relevant model membranes, such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine and egg yolk phosphatidylcholine at room temperature in solution at a resolution in the range of 1 to 2 nm. In addition, two-dimensional arrays were grown directly on these model membranes without any special treatment, and were also imaged by AFM. These results demonstrate the ability of AFM for imaging membrane proteins at high resolution without the need of chemical cross-linking, either within the membrane or to the substratum.
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