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  • Title: Role of pH-sensitive ion channels in regulation of cell volume in opossum kidney cells.
    Author: Mori Y, Kubokawa M, Hagiwara N, Kubota T, Kotera K, Fujimoto M.
    Journal: Jpn J Physiol; 1994; 44 Suppl 2():S81-6. PubMed ID: 7752559.
    Abstract:
    Applying the patch-clamp technique to cultured opossum kidney (OK) cells, we investigated effects of pH on the activation of Cl- channels induced by a hyposmotic shock. The Cl- conductance of cell membranes was measured in 145 mM NMG-Cl solution with the whole-cell configuration. Under control conditions, the Cl- conductance was almost negligible. When the cell was exposed to a hyposmotic medium, the Cl- conductance was remarkably elevated. This conductance change was reversibly inhibited by Cl- channel inhibitors, such as DIDS. The activation (pHi) from 7.3 to 7.0, but not influenced by changing the bath pH (pHe). We conclude that the Cl- channel activation by a hyposmotic challenge is highly sensitive to pHi.
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