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  • Title: [A simple method for evaluation of the lecithin/sphingomyelin ratio in amniotic fluid. Variation in normal and abnormal pregnancies (138 cases) (author's transl)].
    Author: Legrand A, Delattre J, Paolaggi F, Michel L, Tescher M, Girard ML.
    Journal: Pathol Biol (Paris); 1976 Feb; 24(2):160-5. PubMed ID: 775408.
    Abstract:
    The determination of the lecithin/sphingomyelin ratio in amniotic fluid is a useful index for assessing fetal pulmonary maturity. The authors propose a simple and specific method : the amniotic lipids are extracted and thin layer chromatography is carried out in an acetic acid containing medium. The L/S ratio was studied and discussed in 138 normal and pathological cases.
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