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  • Title: Expression in Escherichia coli of the extrinsic 18-kDa protein of photosystem II of spinach.
    Author: Kuwabara T, Takeuchi M, Honda S, Nakajima N, Watanabe A, Kondo N.
    Journal: Plant Cell Physiol; 1995 Apr; 36(3):435-9. PubMed ID: 7757336.
    Abstract:
    A cDNA encoding the precursor for the 18-kDa protein of PSII of spinach was expressed in Escherichia coli. When the cell lysate was incubated at 7 degrees C, the precursor was degraded by proteases of E. coli to a polypeptide of 18 kDa (P18) that consisted of the mature protein moiety plus the last four residues of the transit peptide. P18 was able to reconstitute the water-oxidizing complex of NaCl-treated PSII membranes supplemented with the 23-kDa protein. Moreover, P18 was cleaved by the prolyl endoproteinase of spinach specifically at the Pro-12-Leu-13 bond, as was the authentic 18-kDa protein. These properties of P18 indicate that the present expression system is potentially useful for studies of the substrate specificity of the endoproteinase, as well as of the structure-function relationships of the 18-kDa protein.
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