These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.
Pubmed for Handhelds
PUBMED FOR HANDHELDS
Search MEDLINE/PubMed
Title: GABAA-mediated inhibition and in vitro epileptogenesis in the human neocortex. Author: Avoli M, Louvel J, Drapeau C, Pumain R, Kurcewicz I. Journal: J Neurophysiol; 1995 Feb; 73(2):468-84. PubMed ID: 7760112. Abstract: 1. We made intracellular and extracellular field potential recordings and ion-selective measurements of extracellular Ca2+ concentration ([Ca2+]o) and extracellular K+ concentration ([K+]o) in human neocortical slices that were obtained in the course of epilepsy surgery. Slices were maintained in vitro at 34-35 degrees C and were perfused with Mg(2+)-free artificial cerebrospinal fluid (ACSF). 2. Spontaneous field potential epileptiform discharges (duration = 2.5-80 s) occurred in most of the slices studied (approximately 60%) after 1.5-2 h of perfusion with Mg(2+)-free ACSF. Intracellular recordings from regular-spiking neocortical neurons showed that epileptiform events consisted of large-amplitude (15-30 mV) depolarizing shifts that were capped by bursts of fast action potentials. A decrease in [Ca2+]o (change in [Ca2+]o = 0.02-0.17 mM, 0.07 +/- 0.046 mM, mean +/- SD, from a baseline of 1.8 mM, n = 10 slices) and an increase in [K+]o (change in [K+]o = 0.5-3.8 mM, 1.6 +/- 1.24 mM, from a baseline of 3.25 mM, n = 10) were associated with each epileptiform discharge. 3. The epileptiform activity induced by Mg(2+)-free ACSF was abolished by bath application of antagonists of the N-methyl-D-aspartate (NMDA) receptor. This procedure also blocked the appearance of spreading depression-like episodes. By contrast, the rate of occurrence of epileptiform discharges was not significantly modified by antagonizing non-NMDA receptors. 4. We also observed spontaneous, rhythmic potentials of positive polarity during perfusion of Mg(2+)-free ACSF; the potentials became hyperpolarizing when the neuron membrane was made less negative than -75 mV with intracellular injection of depolarizing current, and they were decreased or abolished during application of the gamma-aminobutyric acid-A (GABAA) receptor antagonist bicuculline methiodide (BMI). The rate of occurrence and/or the amplitude of these presumably GABAA-mediated events decreased approximately 2 s before the onset of each epileptiform discharge. 5. Application of BMI prolonged the epileptiform discharges while decreasing their rate of occurrence. These changes were also accompanied by an increase in the amplitude of the epileptiform field potential DC shift, whereas the concomitant decreases in [Ca2+]o and increases in [K+]o became more pronounced than in control Mg(2+)-free medium (31.2% and 42.8%, respectively, n = 10 slices). 6. Intracellular analysis of regular-spiking neurons in slices that did not generate spontaneous epileptiform discharges after > 2 h of perfusion with Mg2+-free ACSF showed all-or none, variable-latency epileptiform bursts that were induced by high-strength focal extracellular stimuli.(ABSTRACT TRUNCATED AT 400 WORDS)[Abstract] [Full Text] [Related] [New Search]