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  • Title: Adrenoleukodystrophy: the restoration of peroxisomal beta-oxidation by transfection of normal cDNA.
    Author: Shinnoh N, Yamada T, Yoshimura T, Furuya H, Yoshida Y, Suzuki Y, Shimozawa N, Orii T, Kobayashi T.
    Journal: Biochem Biophys Res Commun; 1995 May 25; 210(3):830-6. PubMed ID: 7763255.
    Abstract:
    In order to elucidate the function of ALDP [a protein encoded by the gene responsible for adrenoleukodystrophy (ALD)], normal ALDP cDNA, inserted in an expression vector driven by chicken beta-actin promotor, was transfected into ALD fibroblasts. In a transient expression system, the fatty acid composition did not change even though the ALDP was newly synthesized based on the findings of a western blot analysis. In a stable expression system, 3 cell lines were strongly positive for ALDP. In these cells the level of very long chain fatty acid (C26:0) turned out to be as low as those of the control, while the activities of C24 beta-oxidation, as checked by two different methods, became normal. From these results, it is concluded that ALDP is indispensable for the function of peroxisomal beta-oxidation, and thus the treatment of ALD may be possible by the supplementation of ALDP.
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