These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Protease from the sarcocarp of Trichosanthes bracteata.
    Author: Kaneda M, Uchikoba T.
    Journal: Phytochemistry; 1994 Feb; 35(3):583-6. PubMed ID: 7764589.
    Abstract:
    A protease has been purified from sarcocarp of Trichosanthes bracteata (Lam.) Voigt by four steps of chromatography. Its M(r) was estimated by SDS-PAGE to be ca 67,000. The optimum pH of the enzyme was 11 at 35 degrees using casein substrate. The enzyme was strongly inhibited by di-isopropyl fluorophosphate, but not by EDTA and cysteine protease inhibitors. The oxidized insulin B-chain was cleaved at the peptide bonds of Cys7 (SO3H)-Gly8, Glu13-Ala14, Try16-Leu17 by the enzyme for 1 min. The results indicated that the T. bracteata protease is serine protease, similar to cucumisin from the sarcocarp of melon fruit (Cucumis melo L. var. Prince).
    [Abstract] [Full Text] [Related] [New Search]