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  • Title: Purification of secreted alpha-amylases by immunoaffinity chromatography with cross-reactive antibody.
    Author: Katoh S, Terashima M.
    Journal: Appl Microbiol Biotechnol; 1994 Oct; 42(1):36-9. PubMed ID: 7765818.
    Abstract:
    Two isozymes of rice alpha-amylases expressed and secreted by recombinant yeast were purified by immunoaffinity chromatography by using cross-reactive antibody. Antibodies raised against partially purified barley alpha-amylase adsorbed rice alpha-amylases in fermentation broth by a cross-reaction. By use of these antibodies as ligands, rice alpha-amylases were concentrated and purified to a high degree in one-step immunoaffinity chromatography. Because of the differences in the contaminating impurities between the barley alpha-amylase (antigen) from barley malt and rice alpha-amylases (target protein) secreted from yeast, the high purity of eluted alpha-amylases was attained without the use of highly purified antigen for immunization. Utilization of cross-reactive antibodies in immunoaffinity chromatography is useful for the purification of recombinant proteins in the absence of a sufficient amount and high enough purity of the target proteins to be purified.
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