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  • Title: Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562.
    Author: Wu SQ, Voelkerding KV, Sabatini L, Chen XR, Huang J, Meisner LF.
    Journal: Leukemia; 1995 May; 9(5):858-62. PubMed ID: 7769849.
    Abstract:
    Using fluorescence in situ hybridization (FISH), we were able to demonstrate 22-24-fold amplification of the bcr/abl fusion gene in the human leukemic cell line K-562. About 60% of the amplified sequences are localized to a large acrocentric marker chromosome, with another 30% clustered on a small acrocentric chromosome. In addition to these two masked Ph chromosomes, the remaining bcr/abl fusion genes are located on a der(2) distal to band q33. G- and C-banding analysis revealed similar unique banding patterns in both masked Ph chromosomes and suggests that amplification occurred by tandem duplication of the bcr/abl fusion site. Because the number of bcr/abl fusion genes may be increasing over time, it is critical that researchers using K-562 cells should be aware of this extensive amplification.
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