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  • Title: An improved simple colorimetric method for quantitation of non-transferrin-bound iron in serum.
    Author: Zhang D, Okada S, Kawabata T, Yasuda T.
    Journal: Biochem Mol Biol Int; 1995 Mar; 35(3):635-41. PubMed ID: 7773198.
    Abstract:
    A simple method for direct quantitation of non-transferrin-bound iron (NTBI) in serum is introduced. NTBI was separated from serum by adding excess nitrilotriacetic acid disodium salt (NTA) to serum to form an Fe-NTA complex and then ultrafiltrated using a micro-filter. The NTBI in the ultrafiltrate was quantitated using a bathophenanthroline-based method. The optimal detection condition and several potential confounding factors were investigated. The actual measurements to samples in vivo and in vitro showed that this method is very practical.
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