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  • Title: A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer.
    Author: Goldberg GS, Bechberger JF, Naus CC.
    Journal: Biotechniques; 1995 Mar; 18(3):490-7. PubMed ID: 7779401.
    Abstract:
    We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.
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