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  • Title: Analysis of bile acids and their conjugates using high-pH anion-exchange chromatography with pulsed amperometric detection.
    Author: Chaplin MF.
    Journal: J Chromatogr B Biomed Appl; 1995 Feb 17; 664(2):431-4. PubMed ID: 7780598.
    Abstract:
    Bile acids and their conjugated forms may be separated by anion-exchange chromatography in alkaline media (0.9 M sodium acetate, 0.1 M sodium hydroxide, 15% v/v acetonitrile) on a CarboPac PA-100 column. The effluent was monitored at high sensitivity, with detection limits of less than 10 microM, using a pulsed amperometric detector. Free bile acids and their glyco- and tauro-conjugated forms were separated and detected within 40 min under isocratic conditions.
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