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  • Title: A bisulfite method of 5-methylcytosine mapping that minimizes template degradation.
    Author: Raizis AM, Schmitt F, Jost JP.
    Journal: Anal Biochem; 1995 Mar 20; 226(1):161-6. PubMed ID: 7785768.
    Abstract:
    The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.
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