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  • Title: The occurrence and distribution of GnRH in the brain of Atlantic hagfish, an agnatha, determined by chromatography and immunocytochemistry.
    Author: Sower SA, Nozaki M, Knox CJ, Gorbman A.
    Journal: Gen Comp Endocrinol; 1995 Mar; 97(3):300-7. PubMed ID: 7789745.
    Abstract:
    In Atlantic hagfish (Myxine glutinosa), chromatographic and immunocytochemical evidence showed that the brain contains a gonadotropin-releasing hormone (GnRH)-like molecule that is closely related to lamprey GnRH-III. The chromatographic studies (HPLC) used specific antisera directed against mammalian GnRH and lamprey GnRH-I. In addition to these antisera, other specific antisera were tested in immunocytochemical studies, including chicken GnRH-I, chicken-GnRH-II, salmon GnRH, and lamprey GnRH-III. Using a lamprey GnRH-I antiserum, an early eluting GnRH form coeluted on HPLC with lamprey GnRH-III standard and an unknown form coeluted with the chicken GnRH-II standard. Radioimmunoassay of brain tissue detected GnRH immunoreactivity only when the lamprey GnRH-I antibody was used. A GnRH-like immunoreactivity was also obtained immunocytochemically in the neurohypophysis with the use of antisera against chicken GnRH-II, salmon GnRH, lamprey GnRH-I, and lamprey GnRH-III. These studies indicate that, contrary to earlier reports, hagfish do have a GnRH-like molecule that is more closely related, in terms of immunological determinants, to lamprey GnRH-III than to other currently known vertebrate GnRH molecules.
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