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  • Title: Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes teichomyceticus leuC gene.
    Author: Castelli P, Donadio S, Marinelli F, Borghi A, Sosio M.
    Journal: Gene; 1995 May 26; 158(1):97-100. PubMed ID: 7789819.
    Abstract:
    A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase (IPMI), and the 5' end of a second ORF corresponding to leuD, which encodes the smaller subunit of IPMI. Further subcloning established that Sl strain PC196 is defective in the large subunit of IPMI.
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