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  • Title: Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR.
    Author: Sorg I, Metzler A.
    Journal: J Clin Microbiol; 1995 Apr; 33(4):821-3. PubMed ID: 7790444.
    Abstract:
    A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.
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