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  • Title: Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.
    Author: Ratner PL, Cleary ML, James E.
    Journal: J Virol; 1978 May; 26(2):536-9. PubMed ID: 77913.
    Abstract:
    Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
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