These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Determination of in vivo amino acid neurotransmitters by high-performance liquid chromatography with o-phthalaldehyde-sulphite derivatisation.
    Author: Rowley HL, Martin KF, Marsden CA.
    Journal: J Neurosci Methods; 1995 Mar; 57(1):93-9. PubMed ID: 7791370.
    Abstract:
    The measurement of amino acid neurotransmitters by high-performance liquid chromatography (HPLC) has emerged as a reliable and sensitive method. This paper describes a method which employs electrochemical (EC) detection of amino acid derivatives formed by a reaction with o-phthalaldehyde (OPA) in the presence of sulphite ions. This is discussed in relation to the problems of previously reported methods based on OPA derivatisation. Precise separation of the following 7 amino acid standards is achieved using isocratic elution: serine, glycine, taurine, glutamate, arginine, alanine and GABA, in order of increasing retention time. Total elution time is 25 min. Derivatisation proceeds at room temperature and the derivatives are stable for up to 5 h. This technique has the sensitivity to determine the concentrations of amino acid neurotransmitters in cerebrospinal fluid (CSF) and an in vivo microdialysis method is discussed for the detection of basal and potassium-stimulated levels of gamma-aminobutyric acid and glutamate from rat hippocampus.
    [Abstract] [Full Text] [Related] [New Search]