These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Chloride channels in brown adipocyte plasma membranes: candidates for mediation of alpha 1-adrenergic depolarization?
    Author: Sabanov V, Nedergaard J.
    Journal: Biochem Biophys Res Commun; 1995 Jun 15; 211(2):639-47. PubMed ID: 7794279.
    Abstract:
    The presence of plasma membrane Cl- channels was investigated in brown-fat cells differentiated in culture. Single channel activity was followed by the patch-clamp technique, with an NMDG+ Cl- pipette solution and a NaCl bath solution. Only rarely was putative Cl- channel activity encountered in the cell-attached mode. However, after excision, ion channel activity of two types was observed in inside-out patches: one type represented the earlier observed non-selective cation channel, and one a putative Cl- channel, with the following characteristics: practically non-rectifying current-voltage relationship with a conductance of approximately 50 pS and a reversal potential of approximately 0 in symmetrical Cl- solutions, voltage dependent channel activity (Po approached 0.5 at positive holding potentials), and rapid flickering activity in the open state. It is discussed whether this Cl- channel may be responsible for the initial depolarization phase observed after alpha 1-adrenergic stimulation of brown-fat cells.
    [Abstract] [Full Text] [Related] [New Search]