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  • Title: High-performance liquid chromatographic determination of (S)- and (R)-propranolol in human plasma and urine with a chiral beta-cyclodextrin bonded phase.
    Author: Pham-Huy C, Radenen B, Sahui-Gnassi A, Claude JR.
    Journal: J Chromatogr B Biomed Appl; 1995 Mar 10; 665(1):125-32. PubMed ID: 7795782.
    Abstract:
    The determination of propranolol enantiomers in microsamples of human plasma and urine by HPLC using a chiral stationary phase is described. After extraction from 200 microliters of plasma or urine with racemic alprenolol as internal standard (I.S.), the enantiomers are separated on a beta-cyclodextrin column with a polar organic mobile phase and determined by fluorescence detection. The retention times of I.S. and propranolol enantiomers are about 12-13 min and 16-18 min, respectively. Peak resolutions are 1.4 for I.S. and 2.2 for propranolol. The use of alprenolol as I.S. improves significantly the coefficients of variation (C.V.: 0.6-4.2%). Sensitivity is approximately 1.5 ng/ml per propranolol enantiomer. The assay is applied to pharmacokinetic studies of racemic propranolol in human biological fluids. The (S)-propranolol levels are always higher than the (R)-antipode concentrations in plasma and urine.
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