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  • Title: Mycobacteria in the intestine of Japanese patients with inflammatory bowel disease.
    Author: Suenaga K, Yokoyama Y, Okazaki K, Yamamoto Y.
    Journal: Am J Gastroenterol; 1995 Jan; 90(1):76-80. PubMed ID: 7801954.
    Abstract:
    OBJECTIVES: It is still controversial whether or not a mycobacterial infection may be a cause of Crohn's disease. Mycobacterium paratuberculosis may be very difficult to detect using routine culture techniques. To clarify this, we detected mycobacterial DNA in patients with inflammatory bowel disease. METHODS: IS900 sequences highly specific to M. paratuberculosis and the groEL gene encoding a conserved mycobacterial antigen were studied in colonic mucosa using polymerase chain reaction (PCR). PCR products were analyzed by Southern blot hybridization. RESULTS: IS900 sequences were detected in all (100%) of 10 patients with Crohn's disease, in 11 (61.1%) of 18 patients with ulcerative colitis, and in 14 (87.5%) of 16 control patients with noninflammatory bowel disease. All IS900 positive samples had groEL PCR products. CONCLUSIONS: Our results, on the basis of the prevalence, do not support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.
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