These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Receptor binding of gonadotropin-releasing hormone antagonists that inhibit release of gonadotropin-II and growth hormone in goldfish, Carassius auratus.
    Author: Murthy CK, Wong AO, Habibi HR, Rivier JE, Peter RE.
    Journal: Biol Reprod; 1994 Sep; 51(3):349-57. PubMed ID: 7803606.
    Abstract:
    In goldfish, GnRH stimulates gonadotropin-II (GTH-II) and growth hormone (GH) release. The two native forms of GnRH, salmon GnRH (sGnRH) and chicken GnRH-II (cGnRH-II), bind to two classes of GnRH binding sites: high-affinity/low-capacity sites and low-affinity/high-capacity sites. Our previous in vitro perifusion studies of goldfish pituitary fragments showed that [Ac-delta 3-Pro1, 4FD-Phe2, D-Trp3,6]-mGnRH (analog E), [Ac-delta 3-Pro1, 4FD-Phe2, D-Trp3,6]-sGnRH (analog C), and [Ac-D(2)Nal1, 4Cl-D-Phe2, D-(3)Pal3,6]-cGnRH-II (analog N) inhibited both sGnRH- and cGnRH-II-stimulated GTH-II and GH release. Interestingly, analog C stimulated GH release but not GTH-II release. The objectives of the present study were 1) to test the site of action of GnRH antagonists in goldfish, 2) to test the relationship between receptor binding affinity of antagonists and their in vitro inhibitory potencies and apparent duration of action, and 3) to compare the binding characteristics of analog C with its differential action on GTH-II and GH release. As in previous studies, analog E suppressed sGnRH-stimulated GTH-II and GH release from perifused pituitary fragments. Similarly, analog E suppressed both sGnRH- and cGnRH-II-stimulated GTH-II and GH release from perifused dispersed goldfish pituitary cells, indicating the direct action of GnRH antagonists at the pituitary cell level. In the receptor binding studies, analog E displaced 125I-[D-Arg6, Pro9NHEt]-sGnRH (sGnRH-A) from crude goldfish pituitary membrane preparations in a dose-dependent manner.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]