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  • Title: The elimination of O-linked glycans from glycoproteins under non-reducing conditions.
    Author: Cooper CA, Packer NH, Redmond JW.
    Journal: Glycoconj J; 1994 Apr; 11(2):163-7. PubMed ID: 7804006.
    Abstract:
    A simple procedure is described for the elimination of O-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive beta-elimination of O-glycans.
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