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  • Title: A structural determinant of differential sensitivity of cloned inward rectifier K+ channels to intracellular spermine.
    Author: Fakler B, Brändle U, Bond C, Glowatzki E, König C, Adelman JP, Zenner HP, Ruppersberg JP.
    Journal: FEBS Lett; 1994 Dec 19; 356(2-3):199-203. PubMed ID: 7805837.
    Abstract:
    Large subtype-specific differences in the sensitivity of cloned inward-rectifier K+ channels of the IRK1, BIR10 and ROMK1 subtype to being blocked by intracellular spermine (SPM) are described. It is shown, by site-directed mutagenesis, that the four orders of magnitude larger SPM sensitivity of BIR10 channels compared to ROMK1 channels may be explained by a difference in a single amino acid in the putative transmembrane segment TMII. This residue, a negatively charged glutamate in BIR10, is homologous to the residue in IRK1 and ROMK1 which has previously been shown to change gating properties and Mg2+ sensitivity. Differential block by physiological SPM concentrations is suggested as a major functional difference between subtypes of inward-rectifier K+ channels.
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