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  • Title: [The antibodies obtained by immunization with a peptide identical to a section of the zinc-binding domain of the early growth response 1 factor (EGR-1) interact with a 102-kDa protein from mouse fibroblasts].
    Author: Popov BV, Kaczmarek L.
    Journal: Tsitologiia; 1994; 36(5):453-8. PubMed ID: 7809981.
    Abstract:
    The early growth response (EGR) genes represent a family of proteins whose structure includes zinc finger domain(s) interacting with a specific site in promotors of different genes to regulate their transcriptional activity. Using as an antigen a short sequence RSDHLTTHIR from the middle zinc finger sequence of EGR-1 we have generated a clone of hybridoma cells producing a monoclonal antibody (mAb) that binds a 102 kDa protein, but does not bind EGR-1 protein. The 102 kDa protein consists apparently of 2 polypeptides, of 58 kDa and 44 kDa, and its expression is strongly stimulated in serum-induced mouse fibroblasts.
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