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  • Title: Determination of the antigenic variability of pseudorabies virus field isolates with monoclonal antibodies.
    Author: Matisová J, Lesko J, Gallo J, Subr Z, Sabo A.
    Journal: Acta Vet Hung; 1994; 42(2-3):343-6. PubMed ID: 7810429.
    Abstract:
    Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs with TOP and "K" strains and 7 Slovak field isolates. Only 2 Slovak isolates reacted with all the MAbs, 3 MAbs reacted with TOP and "K" strains. One Slovak isolate gave no reaction with any MAbs.
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